منابع مشابه
Large-scale genomic deletions mediated by CRISPR/Cas9 system
Owing to its simplicity of manipulation and high efficiency, the CRISPR/Cas9 technology has already been achieved as a robust genome engineering tool for generating gene mutations, multiple-gene knockout and knock-in in various species. However, tissues and genomic mosaicism have been widely reported in the gentically modified animals generated from zygotes that were coinjected with Cas9 mRNA a...
متن کاملmodeling loss data by phase-type distribution
بیمه گران همیشه بابت خسارات بیمه نامه های تحت پوشش خود نگران بوده و روش هایی را جستجو می کنند که بتوانند داده های خسارات گذشته را با هدف اتخاذ یک تصمیم بهینه مدل بندی نمایند. در این پژوهش توزیع های فیزتایپ در مدل بندی داده های خسارات معرفی شده که شامل استنباط آماری مربوطه و استفاده از الگوریتم em در برآورد پارامترهای توزیع است. در پایان امکان استفاده از این توزیع در مدل بندی داده های گروه بندی ...
DNA targeting by the type I-G and type I-A CRISPR–Cas systems of Pyrococcus furiosus
CRISPR-Cas systems silence plasmids and viruses in prokaryotes. CRISPR-Cas effector complexes contain CRISPR RNAs (crRNAs) that include sequences captured from invaders and direct CRISPR-associated (Cas) proteins to destroy corresponding invader nucleic acids. Pyrococcus furiosus (Pfu) harbors three CRISPR-Cas immune systems: a Cst (Type I-G) system with an associated Cmr (Type III-B) module at...
متن کاملThe role of Cas8 in type I CRISPR interference
CRISPR (clustered regularly interspaced short palindromic repeat) systems provide bacteria and archaea with adaptive immunity to repel invasive genetic elements. Type I systems use 'cascade' [CRISPR-associated (Cas) complex for antiviral defence] ribonucleoprotein complexes to target invader DNA, by base pairing CRISPR RNA (crRNA) to protospacers. Cascade identifies PAMs (protospacer adjacent m...
متن کاملDisabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein
CRISPR (clustered regularly interspaced short palindromic repeat)-Cas adaptive immune systems are prevalent defense mechanisms in bacteria and archaea. They provide sequence-specific detection and neutralization of foreign nucleic acids such as bacteriophages and plasmids. One mechanism by which phages and other mobile genetic elements are able to overcome the CRISPR-Cas system is through the e...
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ژورنال
عنوان ژورنال: Science
سال: 2019
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.364.6439.448-b